The rack corridor of a finished MushroomWale grow room, before filling
Laboratory Infrastructure

Mushroom Spawn Lab Setup in India

Complete spawn production laboratory with laminar airflow, autoclave, HEPA clean room, and incubation facility. Produce your own Agaricus bisporus, Pleurotus ostreatus, and Calocybe indica spawn from ₹5 lakhs.

GSTIN 09AAZCA0158R1ZP·ICAR-DMR Protocol·Training Included
The short answer

What Is a Mushroom Spawn Lab?

A mushroom spawn lab is a sterile laboratory facility equipped with laminar airflow cabinets, autoclaves, and HEPA-filtered clean rooms for producing mushroom spawn (seed). Spawn is made by inoculating sterilized grain (wheat or jowar) with pure mushroom culture under aseptic conditions. A well-managed lab produces high-quality spawn at ₹20-30/kg vs ₹80-120/kg market price.

Spawn Lab Components

Laminar Airflow Cabinet

HEPA-filtered sterile workspace for inoculation. Class 100 clean air ensures zero contamination during culture transfer.

Autoclave System

121°C, 15 PSI sterilization for grain and media. Horizontal autoclave in 100-200 litre capacity.

HEPA Clean Room

Class 10,000 environment with positive pressure, UV sterilization, and epoxy-coated surfaces.

Climate-controlled infrastructure

Incubation Room

Temperature-controlled (22-25°C) dark room for spawn colonization over 12-15 days.

Grain Processing Area

Washing, boiling, and drying equipment for wheat/jowar grain preparation before sterilization.

Complete Training

5-day hands-on training covering culture handling, sterilization, inoculation, and quality control.

Training programs

Spawn Lab BOQ Checklist

Laminar airflow cabinet (4 ft or 6 ft)
Autoclave (horizontal, 100-200 litre capacity)
Hot air oven for glassware sterilization
HEPA-filtered clean room AC system
UV sterilization lamps (ceiling-mounted)
Incubation room with temperature control
Grain storage bins (SS or food-grade)
Grain boiling and drying equipment
Laboratory glassware set (flasks, petri dishes)
Inoculation tools (loops, needles, spirit lamps)
pH meter and moisture meter
Culture storage refrigerator (2-8°C)
Epoxy-coated clean room flooring
Air lock entry with shoe-change area
Spawn packaging machine (optional)
Cost & return

Spawn Lab Setup Cost & Revenue (2026)

Spawn production is one of the highest-margin businesses in mushroom farming — ₹80-120/kg selling price vs ₹20-30/kg production cost.

SizeCostYieldPayback
Basic spawn lab (500 kg/mo)₹5-10 Lakh₹40K-60K/month revenue8-12 months
Mid-scale (1000 kg/mo)₹10-18 Lakh₹80K-1.2L/month revenue8-10 months
Commercial (2000+ kg/mo)₹20-30 Lakh₹1.5-2.5L/month revenue10-14 months
Integrated farm + lab₹35-50 LakhSaves ₹30-50/kg on spawnSelf-sufficient operation

* Costs exclude building construction, land, and GST @18%.

Calculate Your Exact ROI

Spawn Lab FAQ

The company behind the build

Why Choose MushroomWale

  1. 01

    Engineering Expertise

    Agrimotion Engineering Private Limited is a registered EPC company (CIN U43900UP2023PTC185328, GSTIN 09AAZCA0158R1ZP) based in Firozabad, Uttar Pradesh.

  2. 02

    Proven Track Record

    Turnkey mushroom projects from a 2-room starter to a 20-room commercial farm.

  3. 03

    Bank-Recognized DPRs

    Bank-format DPRs prepared to NABARD, NHB and MIDH norms for loan and subsidy applications.

Bankable DPR · 15-Module Training · Quote in 24h

DPR, Subsidy & Training for Your Spawn Lab Project

Every MushroomWale project ships with a NABARD-compliant DPR and access to our hands-on training curriculum — so you're farm-ready from Day 1.

Bankable DPR & Subsidy Filing

  • NABARD / NHB / MIDH / PMEGP compliant format
  • Itemized BOQ, cash-flow & break-even analysis
  • Subsidy mapping — MIDH or NHB, plus state top-ups
  • Bank loan submission support
Learn about DPR & bank loans

15-Module Hands-On Training

  • Composting (Phase I & Phase II) protocols
  • Spawning, casing & cropping practice
  • Climate control, IPM & post-harvest
  • Based on ICAR-DMR Solan curriculum
View full training curriculum

Get your Spawn Lab quote + DPR within 24 hours

Free site evaluation · GST 18% & transport extra · 5-day quote validity

A finished MushroomWale PUF panel grow room with its door, controller and cooling units
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Ready to Build Your Spawn Lab?

Get complete spawn lab design, equipment list, and installation quote.

  • Turnkey farms built
  • Pan-India delivery
  • Bank-ready DPR